408 



BLOOD AND LYMPH. 



are also absorbed. The variations in the absorption spectrum, 

 with differences in concentration, are clearly shown in the accom- 

 panying illustration from Rollett * (Fig. 174); the thickness of the 

 layer of liquid is supposed to be one centimeter. The numbers 

 on the right indicate the percentage strength of the oxyhemoglobin 

 solutions. It will be noticed that the absorption which takes 



E b 



60 



650 640 630 620 610 600 5Jti 580 570 560 550 5W 5J0| 520 



5/0 500 MO 



,L,I..,,L In 



Fig. 173. Table of absorption spectra (Ziemke and Mutter) : 1, Absorption spectrum 

 of oxyhemoglobin, dilute solution ; 2, absorption spectrum of reduced hemoglobin ; 3, ab- 

 sorption spectrum of methemoglobin, neutral solution; 4, absorption spectrum of met- 

 hemoglobin, alkaline solution ; 5, absorption spectrum of hematin, acid solution; 6, ab- 

 sorption spectrum of hematin, alkaline solution. 



place as the concentration of the solution increases affects the 

 red-orange end of the spectrum last of all. 



Solutions of reduced hemoglobin examined with the spectroscope 



show only one absorption band, known sometimes as the "f-band." 



This band lies also in the portion of the spectrum included between 



the lines D and E; its relations to these lines and the bands of 



* Hermann's " Handbuch der Physiologic," vol. iv., 1880 



