BACTERIOLOGICAL INTRODUCTION 23 



Fig. 13. They are made by pressing a cover glass 

 on the surface of the growth and subsequently 

 staining the adherent organisms. Under a low 

 power (Fig. 13) the general character of the growth 

 is well seen, while a similar preparation seen under 

 a higher magnification (Fig. 8) shows the relation of 

 the individual cells and of the filaments to one 

 another, and displays the mode of growth which 

 results in the production of the peculiar convoluted 

 colonies with fibrillar offshoots which characterise 

 the organism represented. 



The mode of multiplication by fission which 

 characterises the Schizophytes occasionally appears 

 to be supplemented by budding or branching. Such 

 budded or branched forms are shown in Fig. 14, 

 which represents a sub-culture on agar of the 

 B. Leprae isolated by Bordone Uffreduzzi. Similar 

 forms are seen in other bacilli and especially in 

 B. Tuberculosis. How far these may represent 

 forms of reproduction and how far they are merely 

 forms of degeneration is still uncertain. They are 

 certainly found most commonly in old cultures not 

 remarkable for their vitality or virulence. The 

 budding and branching have also been thought 

 to betray a genetic relationship with the yeasts and 

 hyphomycetes. 



