180 TECHNOLOGY OF BACTERIA. 



ilized urine, beef- tea, chicken bouillon, hay infusion, 

 Colm's fluid, etc., and would recommend others who 

 may be inclined to pursue experimental investiga- 

 tions relating to the bacteria to provide them- 

 selves in the same way. For a reserved supply 

 of these and other culture-fluids, flasks containing 

 from two to four fluid ounces will be found of a 

 convenient size. The necks of these flasks are to 

 be drawn out in a powerful flame, so as to form 

 a slender tube the extremity of which can be 

 easily fused in the flame of an alcohol lamp, and 

 which is long enough to permit of its being 

 broken off at the end and resealed several times. 

 The fluid is introduced into these flasks exactly 

 as directed for the smaller ones, viz., by apply- 

 ing heat to the body of the flask, so as to rarefy 

 the enclosed air, and plunging the extremity of 

 the slender neck of the flask, inverted, beneath 

 the surface of the fluid contained in a suitable 

 vessel. These flasks are to be hermetically sealed 

 and sterilized exactly as was directed for the 

 smaller ones. Each flask should have attached 

 to it a label showing the character of its contents 

 and the date of sterilization. 



Culture- Oven. As culture experiments are com- 

 monly conducted at a constant temperature, it is 

 necessary to have a receptacle for the culture- 

 tubes and flasks which can be heated artificially 

 to any desired point, the temperature being regu- 

 lated by a thermostat. 



