226 GERMICIDES 'AND ANTISEPTICS. 



tobacco-infusion (Bucholz). One part in 1,000 

 destroys the spores of Bacillus anthracis in ten 

 minutes (Koch). 



Nitric Acid in the proportion of 1 : 400 destroys 

 the virulence of septicaemic blood time of con- 

 tact, half an hour (S). 



Nitrous Acid. Exp. No. 36, Jan. 22, 1880.- 

 Three children were vaccinated with ivory points 

 which had been exposed for six hours to an at- 

 mosphere containing one per cent of nitrous acid 

 gas (generated by pouring nitric acid on copper 

 filings, and collected over mercury). Result: Vac- 

 cination was unsuccessful in each case, with 

 disinfected points, and successful with the non- 

 disinfected points from the same lot (S). 



Oil of Mustard, in the proportion of 1 : 33,000, 

 prevents the development of the spores of bacilli 

 (Koch.) The development of bacteria in unboiled 

 beef-tea is prevented by 1 : 3,353 ; and 1 : 40 de- 

 stroys the vitality of bacteria in broken-down beef- 

 tea (de la Croix). 



Oil of Turpentine destroys the spores of bacilli 

 in five days, and retards their development in the 

 proportion of 1 : 75,000 (Koch). Turpentine has 

 no action upon the virus of symptomatic anthrax 

 (Arloing, Cornevin, and Thomas). 



Osmic Acid, in one per cent solution, destroys 

 the spores of bacilli in one day (Koch). 



Oxalic Acid, in saturated solution, destroyed the 

 virulence of the fresh virus of symptomatic an- 

 thrax, but had no effect upon dried virus (Arloing, 

 Cornevin, and Thomas). 



