274 BACTERIA IN INFECTIOUS DISEASES. 



have no doubt that you will find that if worked 

 up with salt-solution and injected into a mouse, 

 you will have the spleen after from twenty-four 

 to thirty-six hours enlarged and infiltrated with 

 Bacillus." This scientific prediction was fulfilled 

 to the letter. The tube only contained a fraction 

 of a grain of dried blood. This was rubbed up 

 with a little salt-solution in accordance with the 

 directions given, and a few minims of the solution 

 injected beneath the skin of a recently-captured 

 mouse. The animal died in a little less than 

 thirty-six hours, and its liver and spleen contained 

 an abundance of bacilli. 



A portion of the spleen of this animal was 

 placed in a culture-cell with a little chicken bouil- 

 lon, and kept for twenty-four hours in the cul- 

 ture-oven, at a temperature of 100 Fahr. The 

 following day the culture-fluid was found to con- 

 tain a luxuriant growth of filaments, many of 

 which contained shining oval spores. A fragment 

 of the spleen of the mouse was used to inoculate 

 a small quantity of blood from a healthy rabbit, 

 drawn directly into a sterilized tube. The an- 

 thrax bacillus multiplied abundantly in this blood, 

 growing into long filaments and forming spores, 

 as in the culture in chicken bouillon. On the loth, 

 two minims of this blood-culture were injected 

 into a small rabbit, and a still smaller quantity into 

 another mouse. The mouse died on the following 

 day, and the rabbit on the IGth. Upon post- 

 mortem examination an abundance of bacilli were 



