300 BACTERIA IN INFECTIOUS DISEASES. 



tubercles which are characteristic of the disease. 

 The culture-medium employed was sterilized serum 

 from the blood of the horse or of the sheep. This 

 was inoculated with a bit cut from one of the 

 tumors, due precautions being taken to prevent 

 accidental contamination. The bacillus multiplied 

 abundantly in the course of a few days. Animals 

 of various species were inoculated with pure-cul- 

 tures, and were found to differ as to susceptibility. 

 As a rule, ulcers occurred at the point of inocula- 

 tion, in rabbits, guinea-pigs, mice, etc., which had 

 an indurated base, and the lymphatic glands in 

 the vicinity of these were tumefied and indurated. 

 When the dose was large, inflammation of the tes- 

 ticles, ovaries, and other organs, was liable to occur. 

 Some of the animals died in the course of a few 

 days. In these, bacilli were found which could be 

 propagated by cultivation, but which were smaller 

 than those found in the original material. 



Two horses were inoculated successfully, and one 

 died at the end of fourteen days. Both exhibited 

 characteristic symptoms of the disease. 



In a case of acquired glanders in man, recently 

 studied by Wassilieff, bacilli, resembling those de- 

 scribed by the authors quoted, were found in the 

 nasal secretion, in blood, and in pus from pustules. 

 They were especially abundant in the unripe pus- 

 tules, and nearly all contained four to six spores. 



Evidence of the inoculability of glanders from 

 the horse to the rabbit, and from the rabbit to the 

 ass, has also been presented by Galtier, in a com- 

 munication to the French Academy of Sciences. 



