GONOKRHCEA. 311 



tiplied abundantly in a culture-fluid during the 

 first twenty-four hours after it was inoculated with 

 gonorrhoeal pus. But after forty-eight hours all 

 development ceased, in consequence of the pabu- 

 lum being exhausted, and the micrococci fell to 

 the bottom of the flask. 



" In September, 1882, Bockhart published his experi- 

 ment- in inoculating the gonococci on the sound human 

 urethra! mucous membrane. His description leaves noth- 

 ing to be desired in point of clearness. The subject of 

 the experiment was a forty-six-year-old paralytic, com- 

 pletely anaesthetic, whose death was expected daily. 

 The material used for infection consisted of gonococci 

 grown in fresh infusion of gelatine through four gen- 

 erations. 



" The urethra of the person experimented upon was 

 previously perfectly sound. Forty-eight hours after in- 

 jection there appeared at the meatus urinarius a slight 

 redness, and on pressure a small quantity of mucous se- 

 cretion could be obtained. The symptoms increased, 

 and on the sixth day a typical gonorrhoea was formed, 

 which increased in severity up to the twelfth day, when 

 the man died. During the whole time the characteristic 

 gonococci were found in the abundant secretions." 

 (Quoted from Keyser.) 



The criticism which the writer feels called upon 

 to make in this case, which is thought by Keyser 

 to be very convincing, is that a series of four suc- 

 cessive cultures is not sufficient to insure the ex- 

 clusion of the original material when the cultivation 

 is conducted upon a solid substratum. As multiplica- 

 tion only occurs upon the surface of the culture- 



