GERMICIDES AND ANTISEPTICS. 219 



Carbonic Oxide. Vaccine lymph may endure at 

 least twenty-four hours' exposure to carbonic ox- 

 ide without losing its specific properties (Braid- 

 wood and Yacher). This gas has no effect upon 

 bacteria, which readily develop in it (Hamlet). 



Carbolic Acid, in the proportion of one to two 

 hundred, destroys B. termo and the septic micro- 

 coccus in active growth, while 1 : 25 failed to de- 

 stroy the bacteria in broken-down beef-tea (old 

 stock) ; the micrococcus of pus was destroyed by 

 1 : 225. The development of all of these organ- 

 isms was prevented by the presence in a culture- 

 fluid of 0.2 per cent = 1 : '500 (S). The micro- 

 coccus of swine plague multiplies abundantly in 

 urine containing 1 per cent of carbolic acid, while 

 the micrococcus of fowl-cholera is destroyed by 

 six hours' exposure to a 1 per cent solution (Sal- 

 mon). A 2 per cent solution destroys the bacte- 

 rium of symptomatic anthrax (dried virus), the 

 time of exposure being forty-eight hours (Ar- 

 loing, Cornevin, and Thomas). The multiplica- 

 tion of bacteria in urine is not prevented by 

 1 : 100 (Haberkorn). In egg-albumen develop- 

 ment of bacteria is prevented by 1 : 200 (Ku'hn). 

 One part to 502 prevents the development of 

 bacteria in unboiled meat-infusion; but the bac- 

 teria in broken-down beef-tea are not destroyed 

 by a 10 per cent solution (de la Croix). A 5 per 

 cent solution required two days to arrest the 

 developing power of the spores of Bacillus anthracis, 

 while a 1 per cent solution destroyed the bacilli 



