220 GERMICIDES AND ANTISEPTICS. 



themselves in two minutes. A solution of 1 : 850 

 prevented the multiplication of these bacilli in a 

 suitable culture-medium. Carbolic acid in solu- 

 tion, in oil or in alcohol, is without effect upon 

 the spores of B. anthracis, which germinated after 

 being immersed 110 days and 70 days, respec- 

 tively, in a 5 per cent solution in oil and in alco- 

 hol (Koch). The same author found that car- 

 bolic acid vapor, at 75 C., for two hours, failed 

 to destroy anthrax spores. Chemical combina- 

 tions with other substances were less efficacious 

 than the pure acid. A 5 per cent solution of zinc 

 sulpho-carbolate destroyed anthrax spores in five 

 days ; a 5 per cent solution of sodium phenate, in 

 ten days, merely reduced their power of develop- 

 ment, while sodium sulpho-carbolate failed to do 

 this within the same time. 



Chloral Hydrate failed to kill the micrococcus of 

 pus in the proportion of 10 per cent, but was 

 successful in the proportion of 20 per cent (S). 



Chloroform. A comparatively brief exposure to 

 chloroform vapor entirely sterilizes vaccine lymph 

 (Braid wood and Yacher). Chloroform has no effect 

 upon the fresh virus of symptomatic anthrax 

 (Arloing, Cornevin, and Thomas). Chloroform is 

 inert as regards the destruction of the spores of 

 the anthrax bacillus (Koch). The development 

 of bacteria in unboiled beef-infusion is prevented 

 by 1 : 103; but 1 : 1.22 failed to destroy the bac- 

 teria of broken-down beef-tea (de la Croix). 



Chlorine. Exp. No. 37, Jan. 27, 1880. Four 



