362 BACTERIA IN INFECTIOUS DISEASES. 



bit was still warm, the other had evidently been dead 

 for several hours. The spleen of the first was but 

 slightly enlarged, that of the second was swollen, hard, 

 and dark-colored in patches. No pigment found in 

 either spleen. 



" A culture-flask containing sterilized rabbit bouillon 

 was inoculated with blood from the heart of rabbit No. 

 1. At the end of twenty-four hours the fluid in this 

 flask swarmed with micrococci. A second culture-flask 

 was inoculated from this, a third from the second, and 

 so on to the sixth, twenty-four hours being allowed in 

 each case for the development of the micrococcus. 

 [The flasks were placed in a culture-oven maintained at 

 a temperature of 100 Fahr. For the author's method 

 of manipulation see p. 177.] 



" Exp. No. 2. July 15. Injected twenty-five min- 

 ims of above culture-fluid (sixth) beneath the skin of 

 a half-grown rabbit. Result. This rabbit died during 

 the night of July 18, and upon post mortem examination 

 was found to present the same pathological appearances 

 as in the former experiment, viz., extensive cellu- 

 litis, with effusion of serum swarming with micrococci. 

 The blood also contained the micrococci in abundance ; 

 spleen somewhat enlarged and dark-colored ; no pig- 

 ment found. 



" A new culture was started from the blood of this 

 rabbit by introducing a minute quantity of blood di- 

 rectly from the left auricle into a culture-flask contain- 

 ing sterilized rabbit bouillon. As before, this was carried 

 by successive inoculations from one flask to another to 

 the sixth culture, the culture-flask being in each in- 

 stance placed in an oven at 100 Fahr., for twenty-four 

 hours, for the development of the micrococcus. 



"Exp. No. 3. July 26. Ten minims of above-cul- 

 ture (No. 6) was injected beneath the skin of a half- 



