SEPTIC J2MI A IN RABBITS. 363 



grown rabbit. Result. The animal died at 10 A. M., 

 July 29, and a post-mortem examination was made at 

 once. The subcutaneous cellular tissue was, as usual, 

 infiltrated with serum containing the micrococcus, 

 which was also present in the blood in large numbers. 

 The spleen was very large and dark-colored. A por- 

 tion was removed for microscopical examination, and 

 the remainder left in situ, the animal being so placed 

 that it should be dependent. No pigment was found 

 in the portion first removed, but the presence of black 

 pigment in the portion left in situ was verified the fol- 

 lowing day (removed at 9 A. M.). 



44 The culture-fluid (No. 6) used in experiment No. 3 

 (July 26) was laid aside in an hermetically sealed 

 culture-flask until September 12, when a minute drop 

 was used to inoculate sterilized bouillon in culture-tube 

 No. 7. This, placed in a culture-oven at 100 Fahr. for 

 twenty-four hours, became clouded, and upon micro- 

 scopical examination proved to be pervaded by the 

 identical micrococcus heretofore described and photo- 

 graphed. A drop of culture No. 7 was used to inocu- 

 late culture No. 8, and the next day, this, being also 

 pervaded by the micrococcus, was used in the following 

 experiment : 



" Exp. No. 4. September 14. Injected ten minims 

 of culture No. 8 into a full-grown rabbit. Result. 

 This animal died at 9 A. M., September 15, and a micro- 

 scopical examination made at once demonstrated the 

 presence of the micrococcus in great numbers in the 

 blood and in effused serum in the subcutaneous con- 

 nective tissue. The usual diffuse cellulitis, extending 

 from the point of inoculation, was present ; spleen 

 small, and contained no pigment. 



44 Remarks. This experiment shows that the micro- 

 coccus retained its vitality and its full virulence at the 



