TUBERCULOSIS. 397 



in the tubercular nodules from a freshly cut section of 

 human lung, obtained through the courtesy of Prof. 

 Hirschfelder, from the dead-house of the City and 

 County Hospital, San Francisco. 



" (Ji) But in a similar specimen from another case, 

 repeated examination failed to demonstrate the presence 

 of the bacillus. 



" (i) Culture-experiments have demonstrated that 

 the bacillus in question multiplies upon the surface of 

 sterilized and jellied blood-serum, prepared in the man- 

 ner described by Koch. 



" (y) But in the writer's experiments the bacilli have 

 not penetrated the culture-medium, or extended upon 

 its surface to such an extent as to indicate that multi- 

 plication was at the expense of this jellied blood-serum, 

 which has seemed rather to serve as a moist, supporting 

 surface; multiplication has apparently been at the ex- 

 pense of the tubercular material introduced for the pur- 

 pose of inoculating this culture-medium. The bacilli 

 were not abundant in this material when it was first 

 obtained from the lungs, or from an enlarged lymphatic 

 gland of a rabbit recently killed ; but after remaining in 

 the culture-tube for a fortnight the bacilli were found 

 in enormous numbers, while the cellular elements of 

 the tuberculous material had to a great extent disap- 

 peared." 



Dr. Feltz of Nancy reports that his attempts to 

 cultivate the tubercle bacillus have been entirely 

 unsuccessful, although he claims to have exactly 

 followed the directions given by Koch in his 

 original paper. 



It will be seen from the above quotation that the 

 writer's attempt to cultivate the bacillus was also, 

 practically, a failure ; for it only multiplied in 



