76 THE BACTERIA IN ASIATIC CHOLERA. [CH. 



broadens, particularly near the surface ; the surface itself 

 then shows a drawing inwards or depression of the gelatine, 

 and as development proceeds this pit is converted into a 



L M 



FIG. 25 continued. 



(I) Cultivation of choleraic comma-bacilli in alkaline gelatine peptone broth. 

 (K L and M) Cultivation of choleraic comma-bacilli in alkaline gelatine peptone. 

 In K and L an air-bubble occludes the upper end of the channel of inoculation. 



funnel the mouth of which is occupied by an air-bubble. 

 The rest of the channel of inoculation is a greyish canal 

 filled with translucent liquefied gelatine, and at the bottom 

 of the canal is a whitish precipitate. During the subsequent 



