98 THE BACTERIA IN ASIATIC CHOLERA. [CH. 



kept, like the other monkeys, in a separate stall, uniformly 

 warm and well-ventilated. The food was copious and of the 

 ordinary kind potatoes, rice, and milk. 



Preparations made of the mucus-flakes of the caecum 

 revealed, besides straight thick spore-bearing bacilli, slightly 

 pointed at the ends, large numbers of motile comma-bacilli. 

 In Fig. 30 I have given an accurate representation of a 

 number of these comma-bacilli present in the same place in 

 the mucus-flake. The identity in morphological appearances 

 and in size with the choleraic comma-bacilli is very striking 

 indeed ; there are the same single commas, and g-shaped, 

 circular, and semi-circular forms. I possess a good many 

 preparations (stained and mounted) of the mucus-flakes of 

 the ileum of cases of acute typical cholera in which the num- 

 ber of comma-bacilli is not by any means so great as in 

 the case of this monkey. I have made a number of plate- 

 cultivations from the contents of the caecum of the monkey, 

 but owing to the hot weather then prevailing (June) the 

 above-mentioned straight bacilli had in the course of twenty- 

 four hours so increased in numbers and so pervaded the 

 gelatine that the whole became liquefied and crowded with 

 them. Owing to this the cultivations became altogether 

 useless. In another monkey that died with diarrhoea, the 

 contents of the csecurn were also crowded with comma- 

 bacilli, many of these S- sna ped and spiral ; but these were 

 conspicuously thicker than those in the former case. 



Of other cases of comma-bacilli in the contents of the 

 caecum in monkeys I shall have something to say later on. 



V. In his second paper 1 Koch says he has repeated, 

 with positive results, the experiments made first by Nicati and 

 Rietsch on guinea-pigs (namely, injection of cultures of 

 1 Deutsche med. Woch. 45, 1884. 



