1 64 THE BACTERIA IN ASIATIC CHOLERA. [CH. 



perhaps that they looked a trifle smaller than those in the 

 choleraic mucus-flakes. Cultivations were made with them 

 in six gelatine plates, and in one of these after three days 

 there were no doubt a few colonies which corresponded with 

 those of the choleraic comma-bacilli ; this was proved to be 

 the case after two more days. Cultivations in gelatine tubes 

 and 'Agar-agar tubes yielded growths indistinguishable from 

 the cholera comma-bacilli. In the other plate-cultivations 

 the gelatine was found on the second day liquefied and 

 crowded with the above-mentioned straight bacilli. 



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FIG. 38. SPECIMEN OF MUCUS-FLAKES FROM A MONKEY. 



1. Spiral forms of comma-bacilli. 



2. Couples of comma-bacilli. 



Magnifying-power 600. 



In another monkey, in which the above experiment had 

 been repeated, the animal died during the second day; the 

 loop was found much distended, and filled with watery fluid, 

 bright red, and containing mucus-flakes. On microscopic 

 examination a few comma-bacilli of the same appearance 

 as those in the first animal could be discovered amongst 

 crowds of straight bacilli. Plate-cultivations did not succeed, 

 the straight bacilli multiplied too rapidly, and in the course 



