ix.] OTHER BACTERIA IN CHOLERA. 177 



In a paper, " Abstract of the Results of an Inquiry into 

 the Causation of Asiatic Cholera" (reprinted from the Pro- 

 ceedings of the Royal Society of Edinburgh, 'and published in 

 the Reports from the Laboratory of the Royal College of 

 Physicians, Edinburgh, vol. i. p. 161), Messrs. Neil Macleod 

 and Walter J. Milles state that they have repeated Koch's 

 experiments, and have arrived at the same results. In 

 criticizing my statements they make certain strictures on 

 me which are quite unwarranted. 



On p. 173 they say: "Klein's experiments, in which he 

 gave the opium in other ways than by the peritoneal cavity, 

 and then injected the cholera-bacillus with negative results, 

 are inconclusive, as he never made a control experiment 

 with his material to see whether he was able to produce 

 Koch's results under Koch's conditions." This is in so far 

 an unwarranted statement, as I have made control experi- 

 ments under Koch's conditions with the results described 

 by Koch. After the eighty-five successful experiments 

 recorded by Koch, I do not see the necessity for me or 

 any one else to emphasize the correctness of Koch's 

 observations. What I wished to point out was, that in 

 order to achieve those positive results, viz. multiplication of 

 the comma-bacilli in the intestine and consequent death of 

 the animals, it is necessary to induce a diseased state of the 

 intestine, and this is done by injecting the tincture of opium 

 into the peritoneal cavity. For I showed that if the narcosis 

 be produced otherwise e.g. by subcutaneous injection of 

 opium tincture or watery opium extract, or by intra- 

 peritoneal injection of watery extract of opium no result 

 follows the introduction of cultures of comma-bacilli into 

 the intestine after soda injection. So that, provided the 

 intestine be not injured, which assuredly it is by the 

 injection of tincture of opium or alcohol into the peritoneal 



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