CONTENTS. 



PART I. BACTERIOLOGY. 



CHAPTER I. APPARATUS. 



The Microscope, i; Apparatus for sterilization, 5; Cleaning glassware, 

 8; Concave slides, fermentation tubes, 10. 



CHAPTER II. CULTURE MEDIA. 



Nutrient bouillon, 17; Standardization of reaction, 19; Sugar-free 

 bouillon, 20; Glycerine bouillon, 21; Peptone solution, 21; Nutrient 

 agar, 21; Glycerine agar egg medium, 23; Gelatine, 23; Litmus milk, 

 24; Potato, 25; Blood serum, 25; Blood agar, 26; Bile and faeces 

 media, 26; Culture media for protozoa, 29. 



CHAPTER III. STAINING METHODS. 



Loffler's methylene blue, 33; Carbol fuchsin, 33; Gram's method, 33; 

 Acid-fast staining, 35; Neisser's stain, 36; Capsule staining, 37; Fla- 

 gella staining, 38; Spore staining, 39; Staining of protozoa, 39. 



CHAPTER IV. STUDY AND IDENTIFICATION OF BACTERIA. GENERAL CONSIDERA- 

 TIONS. 

 Methods of isolating bacteria, 41; Classification, 43; Use of keys, 45. 



CHAPTER V. STUDY AND IDENTIFICATION OF BACTERIA. Cocci. 



Key, 49; Streptococci, 50; Sarcinae, 53; Staphylococci, 54; Pneumo- 

 coccus, 55; Gram-negative cocci, 57. 



CHAPTER VI. STUDY AND IDENTIFICATION OF BACTERIA. SPORE-BEARING 

 BACILLI. 



Key, 63; Anthrax, 64; Cultivation of anaerobes, 67; Malignant cedema, 

 69; B. botulinus, 70; B. tetani, 72; B. aerogenes capsulatus, 75. 



CHAPTER VII. STUDY AND IDENTIFICATION OF BACTERIA. BRANCHING, CURV- 

 ING BACILLI. MYCOBACTERIA. CORNYEBACTERIA. 



Acid-fast bacilli, 76; Tubercle bacillus, 78; Leprosy bacillus, 82; 

 Non-acid-fast branching bacilli, 84; B. mallei, 84; B. diphtheriae, 85; 

 Hofman's bacillus, 89; B. xerosis, 89. 



CHAPTER VIII. STUDY AND IDENTIFICATION OF BACTERIA. 



Gram-negative bacilli, Hemophilic bacteria, 91; Influenza bacillus, 92; 

 Friedlander's bacillus, 95; Plague, 95; Eberth, Gartner, and Escherich 

 groups, 99; Typhoid, 100; Dysentery, 105; Chromogenic bacilli, 109. 



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