COLEY'S FLUID in 



bouillon for ten days. This streptococcus culture is now inoculated with B. prodi- 

 giosus, and after another ten days the mixed culture is killed by heat at 60 C. and the 

 sterile product injected. Coley injected about one-twentieth of a c.c. of this vaccine. 

 At present he uses nonnltered, heat sterilized bouillon cultures of a streptococcus 

 obtained either from a case of erysipelas or septicaemia. To this is added material 

 from agar cultures of B. prodigiosus, grown separately and sterilized before adding 

 to the sterilized streptococcus bouillon culture. 



