THE AMCEB^E OF MAN 



2IQ 



which the so-called chromatin-staining methods bring out as reddish 

 areas, we frequently observe smaller aggregations of chromatin-staining 

 material in the cytoplasm. This extranuclear chromatin is supposed 

 to play a part in .the more intricate divisions which such protozoa un- 

 dergo. Food vacuoles and contractile vacuoles are present in many 

 rhizopods. 



Entamoeba coli (Amoeba coli). This is considered by Schaudinn to 

 be a harmless inhabitant of the intestines and its presence in the faeces 

 is not considered of importance. 



FIG. 58. Various protozoa, i, Entamoeba coli; 2, Entamceba histolytica; 3, 

 Leydenia gemmipara; 4, Trichomonas vaginalis; 5, Trichomonas intestinalis; 6, 

 Lamblia intestinalis; 7, flagellated Leishmania donovani; 8, Leishmania donovani 

 in phagocyte; 9, Eimeria stiedae; 10, Isospora bigemina; n, Trypanosoma gambiense; 

 12, Balantidium coli. 



It is now recognized that amoebae of man are not cultivable. When we obtain 

 cultures on the various nutrient poor agar plates, formerly so much used, we find 

 that the amoebae belong chiefly to water amoebae, in particular a Limax. 



The only safe way in recognizing amoebae in stools is to note amoeboid movement. 

 The encysted amcebae, except by the experienced, can scarcely be differentiated 

 from many vegetable cells and especially from large phagocytic cells, of probable 

 endothelial origin. By the use of neutral red in very dilute solution the granular 

 endoplasm will be observed to take up the brick-red stain. 



