CHAPTER XXVII. 



THE URINE. 



MATERIAL for staining is best obtained by centifuging the urine, 

 then pouring off the supernatant urine, then draining the mouth of 

 the centrifuge tube against a piece of filter-paper so that we have only 

 the pus sediment to finally remove with a capillary bulb pipette and 

 make smears. 



The addition of a loopful of egg albumen or blood serum to about twice that 

 amount of urinary sediment gives better results. (See under Staining Methods.) 



Orange 'Yellow Green 



B C D c 



Oxy haemoglobin 



MeJkaemo<globin - alkaline 



Mefhaemoglobin -faintly acid 



Corborv monoxide haemoqlobm 



haemoglobin 



FIG. 98. i, Various absorption bands of spectrum; 2, crystals of glucosazone 

 (Phenylhydrazine sugar test); 3, Cammidge crystals (interacinar type of pancreati- 

 tis); 4, Cammidge crystals (interlobular type of pancreatitis). 



The smear may be stained directly by Wright's method or after fixing by heat 

 with Gram's stain, T. B. stain, or haematoxylin and eosin. The latter is the best for 

 the staining of epithelial cells and animal parasites; the Gram method for bacteria. 



It is frequently difficult to distinguish the spores of moulds from red blood-cells 

 except by measurement and staining reactions. Spores of moulds rarely exceed 

 five microns. 



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