BLOOD PARASITES 353 



about i c.c. blood and put in a centrifuge tube containing 5 c.c. of 3% acetic acid. 

 After the red cells are thoroughly laked centrifuge, pipette off supernatant fluid and 

 dissolve the sediment in 5 c.c. antiformin. When dissolved add 5 c.c. absolute 

 alcohol and centrifugalize for twenty minutes. Smear out the sediment and stain. 



The examination of the blood for the parasites of malaria, filariases, kala-azar 

 and spirillum fevers has been discussed under their respective headings. 



With trypanosomes from human trypanosomiasis, smears from gland juice or 

 cerebrospinal fluid seem more satisfactory to examine than blood smears unless the 

 blood is taken in 5 to 10 c.c. quantities and centrifuged in sodium citrate salt solution. 



The latest method in the diagnosis of trichinosis is to take 5 to 10 c.c. of blood 

 from a vein at the time of the migration of the embryos to the muscles (10 to 20 

 days). This is forced out into a centrifuge tube containing 3% acetic acid, and 

 the sediment examined for trichina larvae. 



