378 APPENDIX 



with gold size. The following is the formula for Kaiser's glycerine jelly: Soak one 

 part of gelatin in six parts of distilled water for two hours. Then add seven parts 

 of glycerine. To the mixture add i % of carbolic acid, warm for fifteen minutes, 

 with constant stirring, and then filter through cotton. 



To Prepare Tape -worms. Wash in salt solution. Wrap around a piece of glass 

 as a glass slide and fix in salt solution containing 2 to 5% of formalin. Then keep 

 the preparation permanently in 70% alcohol. If preferred, the specimen may be 

 run through alcohols and xylol and mounted in balsam. 



Larvae. Mosquito larvae may either be prepared as for small round worms or 

 they may be dropped into 70% alcohol at 60 C. and then passed through alcohols 

 and cleared in xylol and mounted in balsam. Flukes and insects may require treat- 

 ment with hot (60 to 70 C.) solution of 10 to 20% sodium hydrate solution. Then 

 wash thoroughly in water and subsequently pass through alcohols to xylol and mount 

 in balsam. Clove oil or cedar oil clears more slowly, but makes specimens less 

 brittle than does xylol. Another satisfactory method is to drop insects or larvae 

 into acetone at 60 C. and after being in this from one to twelve hours to clear in 

 xylol or clove oil and mount in balsam. 



^ Nematodes. Looss has a method of first washing a small nematode or delicate 

 fluke in salt solution. Then pouring this first salt solution out of the test-tube in which 

 the washing was carried out, to add fresh salt solution, and then an equal amount 

 of saturated aqueous solution of bichloride of mercury. The shaking is easily 

 carried on in the test-tube. After washing in water the worm is passed through 

 alcohols, one strength of which should contain iodine. Clear in xylol and mount in 

 balsam. 



An excellent method is that of Langeron. 



After washing in salt solution fix for a few hours in 5% formalin. Then transfer 

 to lactophenol which has been diluted with an equal amount of water. Allow to 

 remain in this solution for several hours and then transfer to pure lactophenol in 

 which fluid the specimens are to be mounted. Ring with paraffin or with gold size. 

 (To make lactophenol take two parts of glycerine and one part each of distilled 

 water, crystallized carbolic acid and lactic acid.) 



A quick method of preparing small nematodes for examination is to fix them for 

 from two to twelve hours in 5 to 10% formalin, this being heated to 60 C. at the 

 time the worms are dropped into it. Then transfer to the following solution: 



Glucose syrup (glucose, 48; water, 52), 100 c.c. 



Methyl alcohol, 20 c.c. 



Glycerine, 10 c.c. 

 Camphor, q.s. (a small lump for preservation). 



They may be mounted directly in this and the cover-slip ringed with about 60 C. 

 paraffin, followed with gold size. 



Preparations so cleared and mounted in glycerine jelly should also be ringed with 

 paraffin or ome cement. 



Flukes, cestodes, and nematodes are best stained with carmine. The following 

 is a good formula. 



Dissolve, by boiling, 4 grm. carmine in thirty drops HC1 and 15 c.c. water. Then 



