APPENDIX 381 



phthalein solution and titrate. If n c.c. of N/io NaOH were required it would be 

 necessary to add 100 c.c. of water to a volume of 1000 c.c. of the diluted hydrochloric 

 acid. 1000X11 = nooo-f-io= noo. 



Other acid and alkali solutions can be made as for N/io HC1 and N/io NaOH. 



D DISEASES OF UNKNOWN OR NOT DEFINITELY DETERMINED 



ETIOLOGY. 



OF TEMPERATE CLIMATES. 



Acute Articular Rheumatism. Various bacteria have been reported as cause. 



Epidemic Poliomyelitis. Material from the cord of child with the disease 

 when injected subdurally, intravascularly, or into the peritoneal cavity of monkeys 

 produced the disease in the animals inoculated. The virus has been passed through 

 three generations of monkeys (Flexner). 



The virus has been found in the brain, spinal cord, mesenteric and salivary glands 

 of monkeys and may remain in the nasal muscosa of monkeys as long as five months. 

 This would indicate the existence of human chronic carriers. With the possible 

 exception of the rabbit only man and the monkey are susceptible. This would 

 indicate that the virus is directly transferred from man to man. The virus is 

 highly resistant to drying and light. It will remain alive for months in dust. It is 

 not sterilized by pure glycerine during many months of contact. It is possibly 

 transmitted by a biting fly, Stomoxys calcitrans. 



Flexner and Noguchi have recently cultivated the virus of poliomyelitis by 

 employing ascitic fluid to which had been added a fragment of sterile rabbit kidney 

 and nutrient agar, this culture medium being covered with a layer of paraffin oil. 

 The growth is obtained under anaerobic conditions. The minute colonies are 

 composed of globular or globoid bodies from .15 to .3 mikron in diameter. These 

 bodies may be single or in chains or in masses. In older cultures bizarre forms are 

 obtained. Monkeys bave been inoculated with the cultures. 



Foot-and-mouth Disease. Probably due to an ultramicroscopic organism. 



Measles. Cause entirely unknown. Hektoen has shown that blood contains 

 the virus. 



Anderson has found that the virus of measles can pass through a Berkefeld filter 

 and loses its infectivity after heating for 15 minutes at 55 C. In infecting monkeys 

 it was found that the blood of patients with measles was infective only just before 

 and for about twenty-four hours after the appearance of the eruption. Mixed nasal 

 and buccal secretions were infective for monkeys for about forty-eight hours from the 

 time of the eruption. The scales from desquamating cases were not capable of 

 infecting monkeys hence it was thought that measles was not contagious during the 

 period of desquamation. 



Mumps. Herb has implicated a diplococcus. Inoculations into Steno's duct of 

 monkeys successful. 



Rabies. Probably the Negri bodies. 



Roetheln (German Measles). Nothing known. 



Scarlet Fever. Streptococci seem most probable cause (S. anginosus). Mallory 

 has implicated epithelial protozoa. 



