APPENDIX. 421 



in puncture cultivations yellow points are seen in two days, these become 

 confluent and rapidly liquefy the jelly, which remains clear with a yellow 

 deposit below ; it is a comparatively large coccus, occurring in irregular 

 masses or in twos or threes. 



III. There is no growth on gelatine at 22 C. 

 (i) Micrococcus gonorrhoea. Grows on blood serum at 37 C. as a thin 

 greyish yellow layer with moist smooth surface ; organism consists of two 



between them ; it is from 0.8 to I.6/* in length and from 0.6 to 0.8/1 in 

 diameter; unlike most other organisms it is contained within the protoplasm 

 of the tissue cells, and is readily decolorized by Gram's method. 



(2) Diplococcus intracellularis meningitidis . Found in fresh exudation 

 of cases of acute cerebral meningitis. Grows on a mixture of agar-agar and 

 gelatine at the temperature of the body ; the growths in the deeper layers 

 are very small, those on the surface are larger, and are somewhat grey ; at 

 first they are round when seen under the microscope, they then become 

 irregular, are finely granular and yellowish brown, the centre is usually darker 

 than the periphery ; on the surface of agar this organism grows well but not 

 along the track of the needle ; it forms a grey, viscid growth as the various 

 colonies run together ; it only remains virulent for about six days, affects 

 mice, guinea-pigs, rabbits and dogs ; is probably very closely allied to the 

 diplococcus of pneumonia ; grows as a coccus sometimes singly but usually 

 arranged in pairs, fours, or small masses ; in single cocci a line of division 

 may usually be seen ; is almost invariably found within the cells contained 

 in the exudation. 



(3) Micrococcus pyogenes tentiis. An irregular coccus, larger than the 

 staphylococci, and not forming masses; found in a certain proportion of 

 unopened abscesses by Rosenbach; cultivated on agar, chain-like micro- 

 cocci in Endocarditis ulcerosa ; micrococci found in disease of the hands 

 and fingers of butchers and tanners, but not yet fully studied. 



Micrococci have also been described in small-pox pustules and in the 

 various internal organs in the lymph of vaccinal vesicles, in scarlatina by 

 Crooke, and in measles, in diphtheria, in inflammation of membranes of 

 the brain, in influenza (doubtful), in ozoena, in haemophilia neonatorium, 

 in acute yellow atrophy of the liver, and in many other diseased conditions. 



In addition to these may be mentioned Pathogenic micrococci, in the 

 blood of patients suffering from " Clou de Biskra or Bouton d'Alep," which 

 excite gangrene when injected subcutaneously into rabbits, or death sixteen 

 hours after they are injected into the blood. 



The Organism is a Bacillus. 



I. The nutrient gelatine is not liquefied. 



II. The nutrient gelatine is liquefied, see p. 429. 



III. Organisms do not grow on nutrient jelly, and only on 

 other media at higher temperatures in the presence of air, 

 see p. 434. 



IV. Organisms will only grow under conditions of anaero- 

 biosis, see p. 436. 



V. Organism has not yet been artificially cultivated outside 



