APPENDIX. 429 



(3) Bacillus Fitzianus. Obtained from the dust of hay, and supposed to 

 be really a variety of the Bacillus subtilis. The colonies are brownish 

 yellow in colour with a dark opaque centre and sharp outline, those lying on 

 the surface of the gelatine are like brownish yellow gelatinous drops ; 

 organisms are from I to 2ft and upwards in length and about ip, in thick- 

 ness, the longer rods are frequently bent at the ends ; there is distinct spore 

 formation ; sets up sethylic alcoholic fermentation, especially when glycerine 

 is present. 



II. The nutrient jelly is liquefied. 



A. Colonies are white ; nutrient substratum remains uncoloured. 



B. Colonies or nutrient substratum coloured, see p. 433. 



A. Colonies are white ; nutrient substratum remains uncoloured. 



a. Colonies branched or with processes. 



b. Colonies circumscribed without branches, see p. 431. 



a. Colonies branched or with processes. 

 a. Colonies are non-motile. 

 ft. Colonies motile and swarming, p. 430. 



a. Colonies are non-motile. 



(1) Bacillus anthracis, see p. 272. 



(2) Bacillus ramosus liquefaciens. Roundish colonies on plates with 

 radiating processes, the rounded disc looking as if it were surrounded by 

 a zone of hairs ; superficial colonies are oval or pear-shaped ; there^is slight 

 liquefaction around the growth and a deep circular funnel is formed, which 

 is surrounded by concentric rings, which gradually increase in size ; run- 

 ning off from the funnel at right angles are a number of branches longer 

 near the surface and becoming shorter as the deeper layers are reached ; 

 the organism is a medium size slightly motile bacillus with blunted ends. 



(3) Bacillus subtilis (Hay bacillus]. Obtained from hay infusion that has 

 been boiled. Grows on plate cultures as white rounded colonies with radiating 

 processes ; liquefies gelatine on plates rapidly ; along the track of needle 

 causes liquefaction which commences at the surface ; first occurs as small 

 whitish colonies, which under a low power have a yellowish brown colour 

 with the hair-like margin, outside this is a narrow clear zone, beyond which 

 again is a greyish layer composed of radiate lines ; on potatoes and on agar 

 it forms a whitish moist creamy layer, which afterwards becomes somewhat 

 granular and dry ; is dryer and more wrinkled looking on agar than on 

 potatoes. Liquefies blood serum ; grows rapidly about 30 C. ; is strongly 

 rerobic ; is a motile organism about 6p, in length and about 2/z in breadth 

 with slightly rounded ends, it divides and multiplies exceedingly rapidly ; 

 large well defined spores are formed (when the supply of nutrition is 

 gradually cut off), about 1. 2/1 in length and .6p. in breadth. 



(4) Bacillus pneumonicus agilis (or Bacillus of vagus pneumonia of rabbits). 

 Grows on gelatine plates as round dark granular colonies with slightly 

 roughened surface and margins ; after from 20 to 24 hours there are marked 

 movements in the middle of the colonies, and liquefaction takes place rapidly ; 

 in needle cultures in gelatine tubes rapid liquefaction of the medium occurs, 

 and a shalloiv funnel-like space, in which the gelatine is liquefied, is 

 formed ; growth on potatoes, spreads very rapidly over the whole surface as 

 a " chamois " red layer; on blood serum grows much more slowly and only 



