10 GENERAL CONSIDERATIONS 



The above cultural and microscopic characters are very 

 similar to those of B. butyricus y and probably some other 

 organisms, so that it is necessary to make inoculation experi- 

 ments if it is required to accurately differentiate these bacilli, 

 B. enteritidis sporogenes being highly pathogenic, and B. butyricus 

 not pathogenic. 



Fig. i. Bacillus enteritidis sporogenes. Typical milk cultures, 



after 24 hours' incubation at 37 C. 

 (From photographs kindly lent by Dr Klein.) 



Klein recommends that I c.c. of the whey from the milk 

 culture be injected subcutaneously into the groin of a guinea-pig. 

 The animal will usually be found dead within twenty-four hours. 

 On making a post-mortem examination the hairs near the site 

 of inoculation are readily stripped off, the skin underneath being 

 green and gangrenous. Beneath the gangrenous area extensive 

 sloughing of the subcutaneous tissue has taken place, and a con- 

 siderable blood-stained, evil-smelling fluid is present, containing 

 numerous bacilli with the characters given above. The bacilli 



