I/O APPENDIX 



during the heating of the blood together give to the medium 

 some special qualities. 



The plates ought not to be used immediately after their 

 preparation. They should be kept either for several days in the 

 incubator at 37 C. uncovered and face down or for 48 hours at 

 laboratory temperature. 



This medium is useful for the isolation of the cholera 

 spirillum (see Chapter III). 



