70 



ESSENTIALS OF BACTERIOLOGY. 



Inoculation in the Eye. The anterior chamber and the cornea 

 are the two places used. The rabbit is fixed upon a board ; 

 the eyelids held apart and head held still by an assistant. A 

 small cut is made in the cornea, a few drops of cocaine having 



FIG. 39. 



Manner of making intravenous injections in the rabbit. 



first been introduced in the eye. The material is passed 

 through the opening with a small forceps, and with a few strokes 

 of a spoon it is pushed in the anterior chamber. 



For the cornea a few scratches made in the corneal tissue 

 will suffice ; the material is then gently rubbed in. 



Inoculation of the Cerebral Membranes. The skin and 

 aponeurosis cut through where the skull is the thinnest. Then 

 the bone carefully trephined, and the dura exposed. In Rabies 

 inoculation, the syringe containing the hydrophobia virus pierces 

 the dura and arachnoid, and the virus is discharged beneath the 

 latter. 



Intra-Tracheal. The bacteria can be introduced directly into 

 the trachea, thus coming in contact with the lungs. 



Intra-duodenal. Cholera germs are injected into the intes- 

 tines after they have been exposed, by carefully opening the 



