98 ESSENTIALS OF B ACTEK IOLOG Y. 



2. Rinsed in alcohol. 2 to 3 minutes. 



3. Aqueous solution potass, permang. (1 per cent.). 10 

 seconds. 



4. Aqueous solution of sulphuric acid. 2 seconds. 



5. Aq. destil. to wash. 



Numbers three, four, and five repeated, until the section is 

 colorless. Then alcohol, oil of cloves, and Canada balsam as 

 usual. 



De GiacornVs method: 



1. Aniline water fuchsia. 24 hours. 



2. Rinse in dilute tr. ferri clilor. sol. 



3. Decolorize in concentrated tr. ferri chlor. 



4. Wash in alcohol, oil of cloves, Canada balsam, etc. 



For cover-glass preparations, wash in water instead of alcohol. 



Tubercle and lepru bacilli are colored by this method also, 

 but syphilis bacilli become decolorized if washed with acids. 



Puthogenests. No pathological actions have yet been definitely 

 proven. They are found in greater quantities the younger the 

 infection is. 



Bacillus of Glanders. (Bacillus Mallei, Loffler-Shiitz.) 



Origin. In the " farcy buds" or little nodules of the disease, 

 by Loffler and Shiitz in 1882. 



Form. Small slender rods, about the size of the tubercle 

 bacillus. The ends rounded. Never appearing in large collec- 

 tions, usually singly. Spores are present. 



Properties. The rods are very resistant, living in a dried state 

 for three months and longer without any spores present. They 

 are not motile ; possess, however, great molecular vibration. 



Growth. The growth occurs between 25 and 40 C., best 

 at 37 C.; it is very sparse upon gelatine, but on glycerine-agur 

 or blood serum a very abundant growth occurs. 



Colonies. On agar or glycerine-agar there appear in two to 

 three days small white glistening drops, which under microscope 

 seem as round granular masses with an even periphery. 



Stroke Cultures. On glycerine-agar and blood serum small 

 transparent drops of whitish or grayish color, which soon 

 coalesce to form a broad band. 



