PATHOGENIC BACTERIA. 99 



Potato. An amber-colored honey-like growth which gradually 

 turns red. 



Staining. Since the bacillus is very easily decolorized, some 

 special methods have been recommended. 



Loffler^s. (For cover-glass preparations.) 



1. Alkaline methylin blue (Loffler's). a 5 minutes. 



2. Acetic acid with a few drops of tropseoliii. 1 second. 



3. Washed in water. 



For Sections. Instead of tropseolin acetic acid, the following 

 mixture is used : 



] Oxalic acid 5 per cent. . . . gtt. j. 

 Cone, sulphuric, acid. .... gtt. ij. 

 Aq. destill. gij. M. 



The sections are kept in this 5 seconds. 

 Kuhne's method. Coverglaxs. 



1. Warm carbol-blue 2 min. 



2. Decolorized in weak sol. of muriatic acid (10 parts to 500). 



3. Washed in water. 

 Sections of Tissue. 



1. Carbol-blue, ^ hour. 



2. Decolorized in per cent, muriatic acid. 



3. Washed in distilled water. 



4. Dehydrated in alcohol 1 second. 



5. Aniline oil with 6 gtts. of turpentine. 5 min. 



6. Turpentine, xylol, Canada balsam. 



If contrast stain, add 5 gtts. of safranin (Bisniark-brown) to 

 turpentine, and use it after the xylol. 



Pathogenesis. If horses, field mice, or guinea-pigs be inocu- 

 lated subcutaneously, with but a very small quantity of culture, 

 a local affection results, followed some time after by a general 

 disturbance ; ulcers form at the point of inoculation ; little 

 nodules, which then caseate, leaving scars and involving the 

 lymphatics ; metastatic abscesses then occur in the spleen and 

 lungs, and death arises from exhaustion. Cattle, pigs, and rab- 

 bits are not easily affected ; man is readily attacked. 



Manner of Infection. Glanders being a highly contagious dis- 

 ease, it requires but a slight wound to allow it to gain entrance. 



