PLATE IV 



Direct Cultivation of Treponema Pallidum. (Noguehi.) 



FIG. 1. Treponemata pallida from a young pure culture in ascitic agar tissue medium, four days 

 old, at 37 C. Dark field. X 1400. 



FIGS. 2 and 3. The same after two weeks. 



FIG. 4. Treponemata pallida from a chancre (for comparison). X 1400. 



FIG. 5. Treponemata pallida from a pure culture in ascitic agar tissue medium, two weeks at 

 37 C. The pallida in free space show typical morphology. 



FIG. 6. A short pallidum with flagella-like projections at both ends. X 1400. 



FIGS. 7, 8, 9, and 10. An ascitic agar tissue culture (pure) showing various phases of longitudinal 



division V 1 4OO 



