154 



BACTERIOLOGY. 



150 (Behring). Cholera spirilla grown in bouillon con- 

 taining no peptone or only 0.5 per cent, of peptone are 

 destroyed in half an hour by 0.1 per cent, of hydro- 

 chloric acid; grown in 2 per cent, peptone-bouillon 

 their vitality is destroyed in the same time on the 

 addition of 0.4 per cent. HC1. In any case the organ- 

 isms to be tested should all be treated in exactly the 

 same way and the results accompanied by a statement 

 of the conditions under which the tests were made. 



The following table gives the results and methods 

 used in an actual experiment to test the effect of blood - 

 serum upon the disinfecting action of bichloride of 

 mercury and carbolic acid upon bacteria: 



TEST FOR THE DIFFERENCE OF EFFECT OF BICHLORIDE OF 

 MERCURY AND CARBOLIC ACID SOLUTIONS ON ANTHRAX AND 

 TYPHOID BACILLI IN SERUM AND BOUILLON. 



Time. 



A. Serum . . . .2.5 c.c. 

 HgCl 2 sol. 1 : 1000 2.5 c.c. 

 Anthrax thread 



B. Bouillon . . .2.5 c.c. 

 HgCl 2 sol. 1 : 1000 2.5 c.c. 

 Anthrax thread 



C. Serum . . . .2.5 c.c. 

 Carbolic sol. 5 p.ct. 2.5 c.c. 

 Typhoid threads 



D. Bouillon . . .2.5 c.c. 

 Carbolic sol. 5 p.ct. 2.5 c.c. 

 Typhoid threads 



1 8 510208045 h U^ hr 2 s 



Solution equals 



1 in 2000 



bichloride. 



Same. 



Solution equals 

 2% per cent, 

 carbolic acid. 



Same. 



Indicates total destruction of bacteria with no growth in media. 

 + Indicates lack of destruction of bacteria with growth in media. 



