466 BACTERIOLOGY. 



vein of a pure culture of staphylococcus aureus gives 

 rise to a genuine ulcerative endocarditis. It has been 

 further shown by Kibbert that the same result may be 

 obtained without previous injury to the valves by in- 

 jecting into a vein the staphylococcus from a potato 

 culture suspended in water. In his experiments not 

 only the micrococci from the surface, but the super- 

 ficial layer of the potato was scraped off with a steril- 

 ized knife and mixed with distilled water, and the 

 successful result is ascribed to the fact that the little 

 agglomerations of micrococci and infected fragments of 

 potato attach themselves to the margins of the valves 

 more readily than isolated cocci would do. Not infre- 

 quently, also, in intravenous inoculations of young ani- 

 mals there occurs a localization of the injected material 

 in the marrow of the small bones. This may take place 

 in full-grown animals when the bones have been injured 

 or fractured. The experimental osteomyelitis thus pro- 

 duced has been demonstrated to be anatomically analo- 

 gous to this disease in man. With regard to the lesions 

 found in the kidneys after intraperitoneal or intravenous 

 inoculation of cultures of the staphylococcus, it has been 

 found that when injected in considerable quantities the 

 organism may be obtained in cultures from the urine, 

 but not sooner than six or eight hours after the injec- 

 tion, and not until the formation of purulent foci in 

 the kidneys has already occurred. 



The Production of Toxic Substances. The peculiar 

 energetic action of the staphylococcus pyogenes aureus 

 on the tissues of warm-blooded animals would seem 

 to indicate that toxic substances are produced by 

 this organism, which play an important part in its 

 infective properties. Grawitz and De Bary have 



