592 BACTERIOLOGY. 



as since its disco verey, though repeatedly sought for, it 

 has seldom been found by subsequent investigators. 



In 1884, Miller observed a curved bacillus in a hollow 

 tooth, which from its behavior in microscopical prepa- 

 rations, in cultures and animal experiments, is probably 

 identical with the Finkler and Prior spirillum; and 

 other very similar spirilla have been found by others. 



DENEKE'S CHEESE SPIRILLUM. 



Obtained by Deneke (1885) from old cheese, but since 

 then rarely met with. Morphologically and culturally 

 it shows greater similarity to Koch's comma bacillus 

 than the Finkler and Prior spirillum, but can be read- 

 ily differentiated from it also. 



Morphology. Curved rods and long spiral filaments, 

 somewhat more slender than the cholera spirillum, the 

 turns in the spiral threads being lower and closer 

 together. Has a single flagelluin attached to one end. 



Stains with the usual aniline colors. 



Biological Characters. An aerobic and facultative 

 anaerobic, liquefying, motile spirillum. Does not form 

 spores. Upon gelatin plates small, punctiform colonies 

 are formed at the end of twenty-four hours, which when 

 slightly magnified are seen to be circular in shape, with 

 sharply defined border and of a greenish-brown color 

 in the centre and paler toward the margins. Later, 

 when liquefaction has commenced, the sharp contour is 

 often lost. The liquefaction progresses more rapidly 

 than with the cholera bacillus, but not so energetically 

 as with the spirillum of Finkler and Prior. In gelatin 

 stick cultures after forty-eight hours a stocking-like 

 pouch is developed, the spirilla sinking to the bottom 



