GLANDERS BACILLUS. 599 



staining sections, and for decolorizing a mixture con- 

 taining 10 c.c. of distilled water, 2 drops of strong 

 sulphuric acid,, and 1 drop of a 5 per cent, solution of 

 oxalic acid; thin sections to be left in this acid solution 

 for five seconds. 



FIG. 81. 



Glanders bacilli. Agar culture. X 1000 diameters. 



Biological Characters. An aerobic, non-motile bacil- 

 lus, whose molecular movements are so active that they 

 have often been taken for motility. It grows on vari- 

 ous culture media at 37 C. Development takes place 

 slowly at 22 C. and ceases at 43 C. It does not form 

 spores. Exposure for ten minutes to a temperature of 

 55 C., or for five minutes to a 3 to 5 per cent, solution 

 of carbolic acid, or for two minutes to a 1 : 5000 solu- 

 tion of mercuric chloride, was effectual in destroying 

 its vitality. As a rule, the bacilli do not grow after 

 having been preserved in a desiccated condition for a 

 week or two; in distilled water they are also quickly 

 destroyed. The bacillus does not grow in infusions of 



