BA CTERIOL OOY. 



hay, straw, or horse-manure, and it is doubtful whether 

 it finds conditions in nature favorable to a saprophytic 

 existence. It grows well in the incubating oven on 

 glycerin-agar. Upon this medium at the end of twenty- 

 four to forty-eight hours, whitish, transparent colonies 

 are developed, which in six or seven days may attain a 

 diameter of 7 or 8 mm. On blood-serum a moist, 

 opaque, slimy layer develops, which is of a yellowish- 

 brown tinge. The growth on cooked potato is especially 

 characteristic. At the end of twenty-four to thirty-six 

 hours at 37 C. a moist, yellow, transparent layer de- 

 velops; this later becomes deeper in color, and finally 

 takes on a reddish-brown color, and the potato about it 

 acquires a greenish-yellow tint. In bouillon it causes 

 diffuse clouding, with ultimately the formation of a 

 more or less ropy tenacious sediment. Milk is coagu- 

 lated with the production of acid. It grows on media 

 possessing an acid reaction, and both with and without 

 oxygen. 



Pathogenesis. The bacillus of glanders is pathogenic 

 for a number of animals. Among those which are most 

 susceptible are horses, asses, guinea-pigs, cats, dogs, 

 ferrets, moles, and field mice; sheep, goats, swine, rab- 

 bits, white mice, and house mice are much less suscep- 

 tible; cattle are immune. Man is susceptible, and in- 

 fection not infrequently terminates fatally. 



When pure cultures of the bacillus mallei are injected 

 into horses and other susceptible animals true glanders 

 is produced. The disease is characterized in the horse 

 by the formation of ulcers upon the nasal mucous mem- 

 brane, which have irregular, thickened margins, and 

 secrete a thin, virulent mucus; the submaxillary lym- 

 phatic glands become enlarged and form a tumor, which 



