628 APPENDIX. 



dry specimens these bodies stain deeply with methylene- 

 blue, and they are solid or vesicular in form. If the 

 examination be made within twelve to eighteen hours 

 after the chill the hyaline bodies are seen to have 

 grown to occupy one-fourth to one-third of the bodies 

 of the red cells. They are more pigmented, and the 

 corpuscles containing them have become gradually paler 

 and somewhat expanded. The pigment granules, which 

 at first are small, increase in size, and the organisms 

 show very active amoeboid movements. At the end of 

 forty-eight hours they occupy entire corpuscles, are very 

 sluggish in their movements, and look like thin, trans- 

 lucent shells, and are usually devoid of color. Many 

 of the organisms then undergo the remarkable change 

 known as segmentation, which precedes and is asso- 

 ciated with chills and fever. The amoeboid movement 

 ceases as well as that of the pigment granules. The 

 latter gradually collect toward the centres of the amoebae 

 until they are in the form of closely packed, more or less 

 central clumps. The protoplasm becomes more finely 

 granular, and indistinct lines of striation are seen, which 

 begin at the periphery. At this stage the organisms 

 may present the appearance of rosettes. The segmen- 

 tation progresses until the entire protoplasm is divided 

 into twelve to eighteen or twenty spheres. The shell 

 of the corpuscles containing a parasite usually bursts, 

 and the small, rounded, hyaline bodies are set free. 

 Each one of these little bodies consists of a translucent 

 protoplasm, with a central, more highly refractile spot. 

 In stained preparations, during the segmenting process, 

 the reticulum becomes denser and sharper, and then 

 breaks up into fifteen to twenty small spheroidal 

 spores. 



