65 6 APPENDIX. 



smaller slips for issue, two or more scarifiers, a curette, 

 four to six razors for shaving the animals, a razor strop, 

 a pair of large scissors, curved on the flat, for clipping 

 the animals, a burette from which glycerin flows while 

 the vaccine pulp is being ground, burette holder, a 

 Dor ing vaccine grinder, clinical thermometers, to take 

 the temperature of the animals, six to twelve small 

 glass dishes with covers, a hard-rubber syringe, of four 

 ounce capacity, to make suction, absorbent cotton, glass 

 vials and corks, and several pounds of soft glass tubing, 

 three-eighths of an inch in calibre, to store virus emul- 

 sion. There should also be gowns and caps for the 

 attendants. Sodium carbonate, bichloride of mercury, 

 bromine (for a deodorizer), alcohol, and glycerin are 

 the chemicals needed. 



For issue for public vaccinations there are also needed 

 packing-boxes, rubber bands, sheet wadding, needles, 

 and wooden toothpicks (for removing the virus from 

 the vials and rubbing it on the scarifications). 



Yield. The material allowed from the five children 

 should vaccinate at least five calves; it may easily 

 vaccinate fifteen calves. Ten grammes of pulp and two 

 hundred charged slips would be an average yield from 

 a calf, and that, when made up, should suffice to vac- 

 cinate at least fifteen hundred persons. Calves vary 

 immensely in the yield. Of two calves vaccinated in 

 precisely the same way one may furnish material for 

 five hundred vaccinations and the other for ten thou- 

 sand vaccinations. 



The Durability of G-lycerinated Virus in Sealed Tubes. 

 As a result of testing from time to time an immense 

 number of specimens of vaccine, the conclusion has 

 been reached that vaccine properly put up should 



