(558 APPENDIX. 



the calves and the trial vaccinations in young children 

 give a much more reliable basis for judging of the 

 character of the virus than any bacteriological counts 

 of colonies. 



Pathogenic bacteria other than the practically non- 

 virulent skin staphylococci are not found when animals 

 are properly kept and vaccinated. The vaccine pulp 

 and serum mixture is added to two and one-half to 

 three and one-half times its bulk of a mixture consisting 

 of two parts of chemically pure glycerin and one part 

 of water. 



Efficient vaccine should be inoculated in a portion of 

 skin no more than one-eighth inch in diameter. 



Care of the Calves. All bedding is avoided and an 

 exclusively milk diet given; thus much of the otherwise 

 unavoidable dust is done away with. 



