186 LABORATORY MANUAL OF AGRICULTURE 



4. Paper plates or blotters for making germination test. 



5. A cotton sack. 



Directions. Reserve twenty grams of the sample for 

 study and germination test. Pour four liters of water into 

 the crock. Add to the water 30 c.c. of the formaldehyde 

 solution. Put four pounds of oats into a cotton sack large 

 enough to hold ten pounds, and submerge it in the prepared 

 solution. Move the sack around in the solution so that all 

 the grain may become thoroughly wet. Remove after thirty 

 seconds. Empty the sack and spread the grain out on a 

 clean surface to dry. Submerge another four-pound sample 

 in a similar manner and remove after two minutes. Spread 

 it out on a clean surface to dry. Submerge a third four- 

 pound sample for five minutes and place it out to dry. Make 

 a germination test of the untreated sample and each of the 

 treated samples. Use one hundred grains of each for the 

 germination test as shown in Ex. 39. Record the vitality 

 of each sample on the outline form. Seed oats should be 

 thoroughly treated, but not left in the solution long enough 

 to injure their vitality. 



Submerged 30 seconds 



After oats are treated for smut they should not be put in 

 bins or sacks that have smut in them. 



