30 BACTERIOLOGY. 



organisms in pure culture, to cultivate them indefinitely 

 under artificial conditions, to reproduce the lesions by 

 inoculation of these pure cultures into susceptible ani- 

 mals, and to continue the disease at will by continuous 

 inoculation from an infected to a healthy animal. By 

 the methods that he employed he demonstrated a series 

 of separate and distinct diseases that can be produced in 

 mice and rabbits by the injection of putrid substances 

 into their tissues. The disease known as septicaemia of 

 mice ; likewise a disease characterized by progressive 

 abscess-formation, and pyaemia and septicaemia of rab- 

 bits, were among the affections first produced by him in 

 this way. It was in the course of this work that the 

 Abbe system of substage condensing apparatus was first 

 used in bacteriology ; that the aniline dyes suggested by 

 Weigert were brought into general use ; that the isola- 

 tion and cultivation of bacteria in pure culture on solid 

 media were shown to be possible; and that animals 

 were employed as a means of obtaining from mixtures 

 pure cultures of pathogenic bacteria. 



With the bounteous harvest of original and important 

 suggestions that was reaped from Koch's classical series 

 of investigations bacteriology reached an epoch in its 

 development, and at this period modern bacteriolog) 

 may justly be said to have had its birth. 



NOTE. I have presented only the most prominent 

 investigations that will serve to indicate the lines along 

 which the subject has developed. For a more detailed 

 account of the historical development of the work the 

 reader is referred to Loffler's instructive and enter- 

 taining Vorlesungen uber die geschichtliche Entwickelung 

 der Lehre von den Bacterien, upon which I have drawn 

 freely in preparing the foregoing sketch. 



