viii CONTENTS. 



CHAPTER VI. 



PAGES 



Preparation of the tubes, flasks, etc., iu which the media are 



to be preserved 138-141 



CHAPTER VII. 

 Technique of making plates Petri plates, Esmarch tubes, etc. 142-151 



CHAPTER VIII. 



The incubating-oven Gas-pressure regulator Thermo-regu- 



lator Safety burner employed in heating the incubator . 152-159 



CHAPTER IX. 



The study of colonies Their naked-eye peculiarities and 

 their appearance under different conditions Differences 

 in the structure of colonies from different species of bac- 

 teria Stab-cultures Slant-cultures 160-166 



CHAPTER X. 



Methods of staining Impression cover-slip preparations 

 Solutions employed Preparation and staining of cover- 

 slips Staining solutions Staining in general Special 

 staining-methods 107-192 



CHAPTER XI. 



Systematic study of an organism Points to be considered in 

 determining the morphologic and biologic characters of a 

 culture Methods by which the various biologic and chem- 

 ical characters of a culture may be ascertained Facts 

 necessary to permit the identification of an organism as a 

 definite species 193-234 



CHAPTER XII. 



Inoculation of animals Subcutaneous inoculation Intra- 

 venous injection Inoculation in to the great serous cavities 

 and into the anterior chamber of the eye Observation of 

 animals after inoculation 235-257 



CHAPTER XIII. 



Post-mortem examination of animals Bacteriological exami- 

 nation of the tissues Disposal of tissues and disinfection 

 of instruments after the examination Study of tissues and 

 exndates during life 258-266 



