SUBCUTANEOUS INOCULATION OF ANIMALS. 239 



the clamp opened the anterior and posterior wire of each 

 pair falls away from the median line, thereby liberating 

 the animal. To secure the animal it is placed upon its 

 back, the head laid in the cradle formed by the bent 

 wires, the latter are adjusted to the proper position, and 

 all secured by the turn of the single set-screw. Of 

 course, the extremities of the animal are to be secured. 

 This is done by means of cords securely held by a 

 patent fastener made by the Tie Co., of Unadilla, N. Y. 

 These fasteners are in every way more convenient than 

 the elects in common use. An idea of the apparatus is 

 given in Fig. 43. 



A very simple and useful holder for guinea-pigs con- 

 sists of a metal cylinder of about 5 cm. diameter and 

 about 13 cm. long, closed at one end by a perforated 

 cap of either tin or wire netting. Along the side of 

 this box is a longitudinal slit 12 mm. wide that runs for 

 9.5 cm. from within 0.5 cm. of the open extremity of 

 the cylinder. The animal is placed in such a cylinder 

 with its head toward the perforated bottom. It is then 

 easily possible to make subcutaneous inoculation by 

 taking up a bit of skin through the slit in the side of 

 the box, or to make intraperitoneal injection by drawing 

 the posterior extremities slightly from the box and 

 holding them steady between the index and second 

 finger, as seen in Fig. 44. It is also very convenient 

 for use when the rectal temperature of these small ani- 

 mals is to be taken. The manipulations can easily be 

 made without the aid of an assistant. Its construction 

 is seen in Fig. 44. 1 



For ordinary subcutaneous inoculations at the root 



1 Centralblatt fur Bacteriologie und Parasitenkunde, 1895, vol. xviii. 

 p. 530. 



