BACILLUS CHAUVEI. 



551 



has no tendency to form very long threads. (Fig. 

 90, A.) 



It forms spores, and when in this stage is seen to be 

 slightly swollen at or near one of its 

 poles, the location in which the spore FlG - 9i. 



usually appears. (Fig. 90, B.) It is 

 markedly prone to undergo degenerative 

 changes, and involution-forms are com- 

 monly seen not only in fresh cultures, 

 but in the tissues of affected animals as 

 well. 



Though actively motile when in the 

 vegetative stage, it, like all other motile 

 spore-forming bacilli, loses this property 

 and becomes motionless when spores are 

 forming. 



It is strictly anaerobic and cannot be 

 cultivated in an atmosphere in which 

 free oxygen is present. It grows best 

 under hydrogen, and does not grow under 

 carbonic acid. 



The media most favorable to its growth 

 are those containing glucose (1.5 to 2 per 

 cent.), glycerin (4 to 5 per cent.), or some 

 other reducing-body, such as indigo- 

 sodium sulphate, sodium formate, etc. Co i nies of the 



When cultivated upon gelatin plates bacillus of symp- 



f> , -, ! tomatic anthrax, 



in an atmosphere ot hydrogen the col- in deep ge i a tin 

 onios appear as irregular, slightly lobu- culture. (After 



. ,. FRANKEL and 



lated masses. After a short time lique- P PEIFFEB .) 

 faction of the gelatin occurs and the col- 

 ony presents a dark, dense, lobulated and broken centre, 

 surrounded by a much more delicate, fringe-like zone. 



