x CONTENTS. 



CHAPTER VI. 



PAGE 



Preparation of the tubes, flasks, etc., in which the media are to 

 be preserved 109-112 



CHAPTER VII. 



Technique of making plates Esmarch tubes, Petri plates, etc. . 113-124 



CHAPTER VIII. 



The incubating-oven Gas-pressure regulator Thermo-regulator 

 Safety burner employed in heating the incubator 125-132 



CHAPTER IX. 



The study of colonies Their naked-eye peculiarities and their ap- 

 pearance under different conditions Differences in the structure 

 of colonies of different species of bacteria Stab-cultures Slant- 

 cultures 133-138 



CHAPTER X. 



Methods of staining Solutions employed Preparation and stain- 

 ing of cover-slips Preparation of tissues for section-cutting Stain- 

 ing of tissues Special staining-methods 139-176 



CHAPTER XI. 



Systematic study of an organism Points to be considered in iden- 

 tifying an organism as a definite species 177-205 



CHAPTER XII. 



Inoculation ol animals Subcutaneous inoculation ; intravenous 

 injection Inoculation into the great serous cavities, and into the an- 

 terior chamber of the eye Observation of animals after inoculation 206-227 



CHAPTER XIII. 



Post-mortem examination of animals Bacteriological examina- 

 tion of the tissues Disposal of tissues and disinfection of instru- 

 ments after the examination . . 228-233 



