DESCRIBING AN ORGANISM. 205 



tions ? How much of this gas is carbonic acid and how 

 much is explosive ? 



12. At what temperature does it thrive best, and the 

 lowest and highest temperatures at which it will de- 

 velop ? What is its thermal death-point, both by steam 

 and dry-air methods of determining this point ? 



13. What is its behavior when exposed to chemical 

 disinfectants and antiseptics ? Does it withstand dry- 

 ing and other injurious influences, both in the vegeta- 

 tive and spore stages? The germicidal value of the 

 blood-serum of different animals may also be tried 

 upon it. 



14. Its pathogenic powers modes of inoculation by 

 which these are demonstrated; quantity of material used 

 in inoculation; duration of the disease and its symp- 

 toms; lesions produced, and distribution of the bacteria 

 in the inoculated animal ; which animals are susceptible 

 and which immune, and the character of its pathogenic 

 activities ? Variations in virulence, and the probable 

 cause to which they are due. Can they be produced 

 artificially and at will ? 



15. The detection of specific, toxic, and immunizing 

 products of growth. 



16. Its behavior when exposed to the influence of 

 blood-serum of animals immunized from it; also its 

 behavior when mixed with serum from an animal in 

 the height of infection by it. Are the relations be- 

 tween the organism and the serum constant and spe- 

 cific? 



10 



