210 BACTERIOLOGY. 



The animal is placed in such a cylinder with its head 

 toward the perforated bottom. It is then easily pos- 

 sible to make subcutaneous inoculation by taking up a 

 bit of skin through the slit in the side of the box, or 

 to make intraperitoneal injection by drawing the pos- 

 terior extremities slightly from the box and holding 

 them steady between the index and second finger, as 

 seen in Fig. 44. It is also very convenient for use 

 when the rectal temperature of these small animals is to 

 be taken. The manipulations can easily be made with- 

 out the aid of an assistant. Its construction is best 

 seen in Fig. 44. l 



For ordinary subcutaneous inoculations at the root 

 of the tail in mice a simple piece of apparatus consists 

 of a bit of board about 7 x 10 cm. and 2 cm. thick, 

 upon which is tacked a hollow, tapering roll of wire 

 gauze, a truncated cone, about 6 cm. long and about 

 1.5 cm. in diameter at one end and 2 cm. at its other 



FIG. 45. 



Mouse-holder, with mouse in proper position. 



end. This is tacked upon the board in such a position 

 that its long axis runs in the long axis of the board, being 

 equidistant from its two sides. Its small end is placed 



i Centralblatt fur Bakteriologie und Parasitenkunde, Bd. 18, 1895, p. 530. 



