APPEA RANGE OF TUBER OLE BA CILL US. 303 



sible. The masses can only be separated and broken 

 up by grinding in a mortar with the addition of some 

 foreign substance, such as very fine, sterilized sand, 

 dust, etc. 



The cultures are of a dirty-drab or brownish-gray 

 color when seen on serum or on glycerin-agar-agar. 



On potato they grow in practically the same way, 

 though the development is much more limited. They 

 are here of nearly the same color as the potato on which 

 they are growing. When cultivated for a time on 

 potato they are said to lose their pathogenic properties. 



On milk-agar-agar they are of so nearly the same 

 color as the medium that, unless they are growing as 

 the mealy looking masses, considerably elevated above 

 the surface, their presence is less conspicuous than 

 when on the other media. 



In bouillon they grow as a thin pellicle on the sur- 

 face. This may fall to the bottom of the fluid and 

 continue to develop, its place on the surface being 

 taken by a second pellicle. 



Under all conditions of artificial development the 

 cultures of this organism are always very dry and 

 brittle in appearance, though in truth the individuals 

 adhere tenaciously together by a very glutinous sub- 

 stance. 



The tubercle bacillus does not develop on gelatin, 

 because of the low temperature at which this medium 

 must be used. 



MICROSCOPIC APPEARANCE OF THE TUBERCLE 

 BACILLUS. Microscopically the organism itself .is a 

 delicate rod, usually somewhat beaded in its structure, 

 though rarely it is seen to be homogeneous. It is either 

 quite straight or somewhat curved or benf on its long 



