322 BACTERIOLOGY. 



absorb all water with blotting-paper, and stain with two 

 or three drops of 



Carbol-fuchsin 10 c.c. 



Distilled water 100 c.c. 



for thirty minutes; absorb all superfluous stain with 

 blotting-paper, and wash the section three times with 

 0.3 per cent, acetic acid, not allowing the acid to act 

 for more than ten seconds each time. Remove all acid 

 from the section by carefully washing in distilled water; 

 absorb all water by gentle pressure with blotting-paper, 

 and finally, at very moderate heat, or with a small bel- 

 lows (Kiihue), dry the section completely on the slide. 



When dried clear up in xylol, and mount in xylol 

 balsam. 



(6) Transfer sections from alcohol to distilled water; 

 from water to the dilute fuchsin solution, and gently 

 warm (about 50 C.) for fifteen to twenty minutes. 

 Transfer sections from the staining-solution to the slide, 

 absorb all superfluous stain with blotting-paper, and 

 then treat them with 1 per cent, acetic acid from one- 

 half to three-quarters of a minute. Remove all trace 

 of acid with distilled water, absorb all water by gentle 

 pressure with blotting-paper, and then treat the sections 

 with absolute alcohol by allowing it to flow over them 

 drop by drop. For small sections three or four drops 

 are sufficient. Under no circumstances should the 

 alcohol be allowed to act for more than one-quarter of 

 a minute. Clear up in xylol and mount in xylol-balsam. 



In method b the tissues are better preserved than in 

 a, where they are dried. 



Very good preparations are also obtained by the use 

 of Loeffler's alkaline methylene-blue, if care be taken 



