324 BACTERIOLOGY. 



diagnostic aid in detecting the existence or absence of 

 deep-seated manifestations of the disease, the glanderous 

 animal reacting in from four to ten hours to subcuta- 

 neous injections of mallein, while an animal not so 

 affected gives no such reactions. 



It is prepared from old glycerin-bouillon cultures of 

 the glanders bacillus by steaming them for several hours 

 in the sterilizer, after which they are filtered through 

 unglazed porcelain. 



